1Y42
Crystal structure of a C-terminally truncated CYT-18 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.880, 73.210, 56.790 |
| Unit cell angles | 90.00, 111.35, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.17886 |
| Rwork | 0.174 |
| R-free | 0.23541 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ts1 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.621 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 28540 | |
| <I/σ(I)> | 20.7 | 3 |
| Completeness [%] | 97.8 | 89.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
