1Y3O
HIV-1 DIS RNA subtype F- Mn soaked
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2004-11-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.894 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 27.341, 117.358, 94.672 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.700 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xp7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 7799 | |
| <I/σ(I)> | 14.3 | 2.28 |
| Completeness [%] | 96.5 | 99 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 310 | PEG8000, Ammonium sulfate, MgCl2, Na Cacodylate, spermine, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 310K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG8000 | ||
| 10 | 1 | 2 | Na Cacodylate | ||
| 2 | 1 | 1 | Ammonium sulfate | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | Na Cacodylate | ||
| 5 | 1 | 1 | spermine | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | PEG8000 | ||
| 8 | 1 | 2 | Ammonium sulfate | ||
| 9 | 1 | 2 | MgCl2 |
