1Y2T
Crystal structure of the common edible mushroom (Agaricus bisporus) lectin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-24 |
Detector | MARRESEARCH |
Wavelength(s) | 1.00 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 91.935, 96.681, 75.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.940 - 1.500 |
R-factor | 0.18887 |
Rwork | 0.187 |
R-free | 0.20298 |
Structure solution method | MIR |
RMSD bond length | 0.005 |
RMSD bond angle | 0.903 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.500 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 53757 | |
<I/σ(I)> | 7.9 | 7.1 |
Completeness [%] | 99.5 | 97.6 |
Redundancy | 6.4 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | Sodium formate, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |