1Y20
Crystal structure of the NR1 ligand-binding core in complex with ACPC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2004-02-19 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.393, 72.634, 96.263 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.960 - 1.400 |
R-factor | 0.201 |
Rwork | 0.199 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.400 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.087 | 0.330 |
Number of reflections | 56029 | |
<I/σ(I)> | 20 | 3.6 |
Completeness [%] | 96.6 | 100 |
Redundancy | 5.3 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | PEG, pH 6., VAPOR DIFFUSION, HANGING DROP, temperature 277K |