1Y1X
Structural analysis of a homolog of programmed cell death 6 protein from Leishmania major Friedlin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-3 |
| Synchrotron site | SSRL |
| Beamline | BL11-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97461 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 86.754, 92.945, 52.137 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.470 - 1.950 |
| R-factor | 0.1898 |
| Rwork | 0.187 |
| R-free | 0.23277 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Partial model from MAD experiments |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.392 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 33506 | |
| <I/σ(I)> | 8.8 | 2 |
| Completeness [%] | 94.2 | 72.7 |
| Redundancy | 5.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Lithium sulfate, HEPES, magnesium sulfate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
