1Y1T
Crystal Structure of the Uridine Phosphorylase from Salmonella Typhimurium at 1.77A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8126 |
| Spacegroup name | H 3 |
| Unit cell lengths | 150.820, 150.820, 47.840 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.400 - 1.770 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sj9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data scaling software | XDS |
| Phasing software | MOLREP (V. 7.3) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.400 | 1.860 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.066 | 0.330 |
| Number of reflections | 38496 | |
| <I/σ(I)> | 15.81 | 4.46 |
| Completeness [%] | 95.8 | 81.6 |
| Redundancy | 1.77 | 1.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 295 | PEG 6000, SODIUM-ACETATE TRIHYDRATE, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
