1Y1E
human formylglycine generating enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-05-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 61.956, 110.014, 43.474 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.800 - 1.730 |
| R-factor | 0.1964 |
| Rwork | 0.194 |
| R-free | 0.24232 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | unpublished model of the FGE paralogue pFGE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.357 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.800 | 1.780 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Number of reflections | 30898 | |
| <I/σ(I)> | 17.3 | 1.6 |
| Completeness [%] | 97.0 | 72.2 |
| Redundancy | 2.9 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | Tris/HCl, CaCl2, PEG4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
