1Y13
Structural Analysis of Plasmodium falciparum 6-pyruvoyl tetrahydropterin synthase (PTPS)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979029 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 103.319, 103.319, 131.111 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.19724 |
| Rwork | 0.195 |
| R-free | 0.23481 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.409 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 36614 | |
| <I/σ(I)> | 10.6 | 2.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.4 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 298 | PEG 1000, HEPES, Ammonium Chloride - Reservoir: 2.2 M Lithium Chloride, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
