1Y0X
Thyroxine-Thyroid Hormone Receptor Interactions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-01-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.09720 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 68.790, 68.790, 130.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 59.760 - 3.100 |
| R-factor | 0.225 |
| Rwork | 0.220 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | TRb-TRIAC |
| RMSD bond length | 0.040 |
| RMSD bond angle | 2.780 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | |
| Number of reflections | 11829 | |
| Completeness [%] | 71.6 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 279 | sodium acetate, cacodylate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
