1Y0K
Structure of Protein of Unknown Function PA4535 from Pseudomonas aeruginosa strain PAO1, Monooxygenase Superfamily
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2004-09-30 |
Detector | SBC-2 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 70.422, 53.918, 55.583 |
Unit cell angles | 90.00, 119.41, 90.00 |
Refinement procedure
Resolution | 48.390 - 1.750 |
R-factor | 0.188 |
Rwork | 0.186 |
R-free | 0.22200 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.167 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.800 |
High resolution limit [Å] | 1.750 | 1.740 |
Rmerge | 0.050 | |
Number of reflections | 18416 | |
Completeness [%] | 98.2 | 86.5 |
Redundancy | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 298 | 1.5M Na Acetate, 0.1M Na Cacodylate, ph 6.5, VAPOR DIFFUSION, temperature 298K, pH 6.50 |