1Y0E
Crystal structure of putative ManNAc-6-P epimerase from Staphylococcus aureus (strain N315)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-19 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97951 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.961, 76.283, 183.661 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 91.670 - 1.950 |
R-factor | 0.17933 |
Rwork | 0.177 |
R-free | 0.21564 |
Structure solution method | SAD |
RMSD bond length | 0.037 |
RMSD bond angle | 2.337 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.142 | 0.539 |
Number of reflections | 54814 | |
<I/σ(I)> | 29.7 | 3.21 |
Completeness [%] | 98.7 | 87.5 |
Redundancy | 19.1 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | NaH2PO4, K2HPO4, imidazole, NaCl, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |