1Y0C
T-to-THigh Quaternary Transitions in Human Hemoglobin: alphaY140F deoxy low-salt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-06-27 |
| Detector | SDMS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 97.100, 99.500, 66.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| Rwork | 0.169 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xxt |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.026 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.094 | 0.150 |
| Number of reflections | 29663 | |
| <I/σ(I)> | 7.4 | 1.9 |
| Completeness [%] | 97.9 | 89.8 |
| Redundancy | 8.1 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 10% PEG 6000, 10 mM potassium phosphate, 100 mM potassium chloride, 3 mM sodium dithionite, 10 mg/ml Hb, pH 7.0, batch, temperature 298K |
