1Y0B
Crystal Structure of Xanthine Phosphoribosyltransferase from Bacillus subtilis.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2003-02-18 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97981,0.97995, 0.990 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.503, 115.902, 73.985 |
| Unit cell angles | 90.00, 94.30, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.1807 |
| Rwork | 0.178 |
| R-free | 0.22818 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.853 |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.230 | 1.846 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 58399 | |
| Completeness [%] | 88.5 | 49.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | Bis-tris, NaCl, PEG3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
