1Y08

Structure of the Streptococcal Endopeptidase IdeS, a Novel Cysteine Proteinase with Strict Specificity for IgG

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-09
Detector	MARRESEARCH
Wavelength(s)	0.97910, 0.95, 0.97935, 1.05
Spacegroup name	P 21 21 2
Unit cell lengths	63.371, 86.829, 57.664
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.930
Rwork	0.196
R-free	0.23500
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.370
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.000
High resolution limit [Å]	1.930	1.930
Rmerge	0.063	0.266
Number of reflections	24440
Completeness [%]	99.3	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293.15	PEG 2000 MME, ammonium sulfate, sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
1	VAPOR DIFFUSION, SITTING DROP	4.5	293.15	PEG 2000 MME, ammonium sulfate, sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K