1Y02
Crystal Structure of a FYVE-type domain from caspase regulator CARP2
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.28 |
Spacegroup name | P 31 |
Unit cell lengths | 51.196, 51.196, 38.564 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.21 |
Rwork | 0.206 |
R-free | 0.23380 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.370 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 12241 | |
Completeness [%] | 97.3 | 85.2 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | HEPES, Na, K, Tartrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |