1XZO
Identification of a disulfide switch in BsSco, a member of the Sco family of cytochrome c oxidase assembly proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97960 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 68.033, 68.033, 191.733 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.200 - 1.702 |
| R-factor | 0.24088 |
| Rwork | 0.238 |
| R-free | 0.28935 |
| Structure solution method | SAD |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.388 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.550 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 54348 | |
| Completeness [%] | 0.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG5K MME, CdCl2, CaCl2, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
