1XZ6
Mutant ABO(H) blood group glycosyltransferase A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 2003-10-01 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 52.900, 150.900, 79.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.550 |
R-factor | 0.222 |
Rwork | 0.209 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.040 | |
Number of reflections | 29971 | |
<I/σ(I)> | 15.5 | |
Completeness [%] | 99 | |
Redundancy | 4.43 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 30 mM sodium acetate, 5 mM MnCl2, 10% (w/v) PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 20K |