1XZ3
Complex of apoferritin with isoflurane
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0722 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.510, 181.510, 181.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.750 |
| R-factor | 0.1804 |
| Rwork | 0.179 |
| R-free | 0.21046 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1gwg |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.185 |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.820 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.064 | 0.431 |
| Number of reflections | 25454 | |
| <I/σ(I)> | 16.6 | 2.2 |
| Completeness [%] | 95.3 | 78.1 |
| Redundancy | 10.4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | cadmium sulfate, HEPES, sodium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
