1XZ2

wild-type hemoglobin deoxy no-salt

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1994-03-07
Detector	SDMS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	83.900, 112.100, 63.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.17136
Rwork	0.168
R-free	0.20094
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hbb
RMSD bond length	0.014
RMSD bond angle	1.491
Data reduction software	SDMS
Data scaling software	SDMS
Phasing software	X-PLOR
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	1.930	1.930
Rmerge	0.059	0.185
Number of reflections	44690
<I/σ(I)>	12.4	2.9
Completeness [%]	96.6	83.2
Redundancy	9	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	batch	7	298	12% PEG 1450, 10 mg/ml Hb, pH 7.0, batch, temperature 298.0K