1XYZ

A COMMON PROTEIN FOLD AND SIMILAR ACTIVE SITE IN TWO DISTINCT FAMILIES OF BETA-GLYCANASES

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Detector technology	IMAGE PLATE
Collection date	1993-11
Detector	MARRESEARCH
Wavelength(s)	0.92
Spacegroup name	P 1
Unit cell lengths	47.100, 51.100, 70.740
Unit cell angles	100.54, 83.79, 101.64

Refinement procedure

Resolution	10.000 - 1.400
R-factor	0.183
Rwork	0.183
R-free	0.22100
RMSD bond length	0.015
RMSD bond angle	23.200 *
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	1.400 *
Rmerge	0.083
Total number of observations	343930 *
Number of reflections	117046 *
Completeness [%]	96.4
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *	24 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	reservoir	PEG2000	20 (%)
4	1	reservoir	calcium acetate	0.2 (M)
5	1	reservoir	Tris-HCl	0.1 (M)