1XYP

STRUCTURAL COMPARISON OF TWO MAJOR ENDO-1,4-BETA-XYLANASES FROM TRICHODREMA REESEI

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-07-20
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	81.820, 60.900, 38.130
Unit cell angles	90.00, 94.43, 90.00

Refinement procedure

Resolution	8.000 - 1.500
R-factor	0.189
Rwork	0.189
RMSD bond length	0.008
RMSD bond angle	27.300 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.500 *
Rmerge	0.086
Total number of observations	90097 *
Number of reflections	39973 *
Completeness [%]	66.0 *
Redundancy	2.25

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.2 *		Torronen, A., (1993) J. Mol. Biol., 233, 313. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	sodium acetate	50 (mM)
3	1	drop	ammonium sulfate	25 (%)
4	1	reservoir	ammonium sulfate	25 (%)