1XYI
Hyperthermophile chromosomal protein Sac7d double mutant Val26Ala/Met29Ala in complex with DNA GCGATCGC
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL17B2 |
| Synchrotron site | NSRRC |
| Beamline | BL17B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-11-28 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.0717 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.780, 47.270, 52.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.450 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.25500 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.047 | 0.395 |
| Number of reflections | 17190 | |
| <I/σ(I)> | 33.7 | 3.5 |
| Completeness [%] | 99.4 | 99.1 |
| Redundancy | 5.4 | 4.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | Tris, PEG400, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Tris | ||
| 2 | 1 | 1 | PEG400 | ||
| 3 | 1 | 2 | Tris | ||
| 4 | 1 | 2 | PEG400 |
