1XYH
Crystal Structure of Recombinant Human Cyclophilin J
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2004-06-26 |
Detector | BRUKER |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 41.309, 41.309, 172.116 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.510 - 2.600 |
R-factor | 0.175 |
Rwork | 0.175 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cpl |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.510 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.084 | 0.372 |
Number of reflections | 5560 | |
Completeness [%] | 95.9 | 92.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | Tris-HCl, PEG8000, DMSO, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |