1XYG

X-RAY STRUCTURE OF GENE PRODUCT FROM ARABIDOPSIS THALIANA AT2G19940

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-24
Detector	MAR CCD 165 mm
Wavelength(s)	0.97167
Spacegroup name	P 1 21 1
Unit cell lengths	84.597, 107.067, 85.754
Unit cell angles	90.00, 118.88, 90.00

Refinement procedure

Resolution	27.540 - 2.190
Rwork	0.187
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vkn
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP (8.2.01version5.0:04/07/04)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.540	50.000	2.250
High resolution limit [Å]	2.190	5.420	2.200
Rmerge	0.059	0.028	0.254
Total number of observations		4629	3832
Number of reflections	66910
<I/σ(I)>	15.5		3.8
Completeness [%]	97.9	99.2	84.6
Redundancy	3.8		3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	10 MG/ML PROTEIN, 23% PEG2K, 0.200 M TETRAMETHYL AMMONIUM CHLORIDE, 0.100 M MOPS, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K