1XXR
Structure of a mannose-specific jacalin-related lectin from Morus Nigra in complex with mannose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-06-27 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9102 |
Spacegroup name | P 65 |
Unit cell lengths | 110.908, 110.908, 160.021 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.970 - 2.000 |
R-factor | 0.191 |
Rwork | 0.191 |
R-free | 0.21100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.045 | 0.189 |
Number of reflections | 70972 | |
<I/σ(I)> | 21.31 | 4.92 |
Completeness [%] | 89.8 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | ammonium sulphate, imidazole buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |