1XXP
Yersinia YopH (residues 163-468) C403S binds phosphotyrosyl peptide at two sites
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Detector | UCSD MARK III |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 54.170, 71.090, 47.860 |
Unit cell angles | 111.80, 90.00, 109.70 |
Refinement procedure
Resolution | 20.000 - 3.000 |
Rwork | 0.181 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yts |
RMSD bond length | 0.008 |
RMSD bond angle | 1.177 |
Data reduction software | SDMS |
Data scaling software | SDMS |
Phasing software | X-PLOR |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 3.000 |
Rmerge | 0.118 |
Number of reflections | 10084 |
Completeness [%] | 86.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 10-12 mg/ml protein + 1 mM phosphopeptide. Precipitant: 18-24% polyethylene glycol (PEG) 8000, 50-100 mM NaCl, 0.1% beta-mercaptoethanol, 100 mM TrisCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |