1XXJ
Urate oxidase from aspergillus flavus complexed with 5-amino 6-nitro uracil
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 291 |
Detector technology | IMAGE PLATE |
Collection date | 2003-11-30 |
Detector | MARRESEARCH |
Wavelength(s) | 0.972 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 126.245, 142.265, 81.322 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.800 |
Rwork | 0.189 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wrr |
RMSD bond length | 0.012 |
RMSD bond angle | 1.108 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.880 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 36394 | |
<I/σ(I)> | 16.8 | 10.1 |
Completeness [%] | 98.7 | 98.4 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 8.5MG/ML PROTEIN, 0.2MG/ML DIAMINOURACIL, REM 5-7%(W/V) PEG 8000, 100MM TRIS/HCL, PH 8.0, NACACODYLATE 100mM pH 7.0, 1mM DTT, 1mM cymelarsan, pH 8.00, VAPOR DIFFUSION, SITTING DROP, temperature 291K |