1XXG
Crystal Structure of Staphylococcal Enterotoxin G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-10 |
Detector | ADSC QUANTAM Q315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 70.738, 70.738, 111.898 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.700 - 2.200 |
R-factor | 0.231 |
Rwork | 0.231 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3sbb |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.700 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 15015 | |
Completeness [%] | 99.9 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG 4000, ammonium sulphate, sodium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |