1XWR
Crystal structure of the coliphage lambda transcription activator protein CII
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Wavelength(s) | 0.9836, 0.9841, 0.9949, 0.9733 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 63.907, 106.794, 119.784 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.560 |
R-factor | 0.25 |
Rwork | 0.250 |
R-free | 0.28600 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.590 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.068 | 0.710 |
Number of reflections | 13650 | |
<I/σ(I)> | 22.4 | 2.1 |
Completeness [%] | 99.8 | 99.4 |
Redundancy | 6.5 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 298 | PEG 3350, isopropanol, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 298 | PEG 3350, isopropanol, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 298 | PEG 3350, isopropanol, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |