1XWM
The crystal structure of PhoU (phosphate uptake regulator), Structural genomics
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-19 |
Detector | SBC-2 |
Wavelength(s) | 0.9795, 0.94656 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 61.172, 61.172, 121.264 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.590 - 2.500 |
R-factor | 0.243 |
Rwork | 0.243 |
R-free | 0.33400 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.300 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.104 | 0.667 |
Number of reflections | 15295 | |
<I/σ(I)> | 22.6 | 1.87 |
Completeness [%] | 99.7 | 98.6 |
Redundancy | 8.5 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 298 | 16% PEG3350, 0.2M ammonium tartrate, 0.1M Bis-Tris buffer, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |