1XWL

BACILLUS STEAROTHERMOPHILUS (NEWLY IDENTIFIED STRAIN AS YET UNNAMED) DNA POLYMERASE FRAGMENT

Replaces:  1BDP

Experimental procedure

Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	1996-12
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	87.106, 93.868, 104.577
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.254
Rwork	0.254
R-free	0.29100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ORIGINAL 1BDP COORDINATES
RMSD bond length	0.010
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.038 *	0.182 *
Number of reflections	89072
<I/σ(I)>	16.2	3.2
Completeness [%]	94.3	83.1
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.8	18 *	pH 5.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ammonium sulfate	55 (%(v/v)sat)
3	1	reservoir	MES	100 (mM)
4	1	reservoir	MPD	2.5-7.0 (%(v/v))
5	1	reservoir	sodium azide	0.02 (%(w/v))