1XWJ
Vinculin head (1-258) in complex with the talin vinculin binding site 3 (1945-1969)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.979625, 0.972447, 0.979404 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 52.173, 72.434, 96.372 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.950 - 2.600 |
Rwork | 0.241 |
R-free | 0.28100 |
Structure solution method | MAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.099 | 0.211 |
Number of reflections | 11765 | |
Completeness [%] | 92.2 | 78.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1M NaH2PO4, 1M K2HPO4, 0.1M CAPS, pH 10.9, 3% Hexanediol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |