1XWG
Human GST A1-1 T68E mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.996 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.077, 90.570, 51.489 |
| Unit cell angles | 90.00, 93.06, 90.00 |
Refinement procedure
| Resolution | 67.000 - 1.850 |
| R-factor | 0.16944 |
| Rwork | 0.164 |
| R-free | 0.21809 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gsd |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.510 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 38714 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | PEG4 000, DTT, Tris-HCl, MPD, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
