1XWC

Drosophila thioredoxin, reduced, P6522

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 65 2 2
Unit cell lengths	52.954, 52.954, 152.939
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.300
Rwork	0.221
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1auc
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.400
High resolution limit [Å]	2.300	2.300
Number of reflections	5951
<I/σ(I)>	12.9
Completeness [%]	95.8	95.6
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	298	ammonium sulfate, pH 8.5, VAPOR DIFFUSION, temperature 298K