1XW5
Human glutathione s-transferase M2-2 (E.C.2.5.1.18)complexed with glutathione, monoclinic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-11 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.200, 49.790, 92.040 |
| Unit cell angles | 90.00, 94.27, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gtu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.064 | 0.017 |
| Number of reflections | 38664 | |
| <I/σ(I)> | 11.6 | 2.2 |
| Completeness [%] | 89.3 | 43.3 |
| Redundancy | 7.7 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 290 | 20% PEG4000, pH 6.50, VAPOR DIFFUSION, temperature 290K |
