1XW3
Crystal Structure of Human Sulfiredoxin (Srx)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-08 |
| Detector | RIGAKU SATURN 92 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 68.057, 68.057, 51.302 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.690 - 1.650 |
| R-factor | 0.21584 |
| Rwork | 0.214 |
| R-free | 0.25817 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SEMET MODEL |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.566 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.700 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.039 | 0.240 |
| Number of reflections | 16690 | |
| <I/σ(I)> | 27.1 | 3.9 |
| Completeness [%] | 98.9 | 92.3 |
| Redundancy | 7.22 | 2.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | MPD, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.50 |
