1XW3
Crystal Structure of Human Sulfiredoxin (Srx)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-08 |
Detector | RIGAKU SATURN 92 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 68.057, 68.057, 51.302 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.690 - 1.650 |
R-factor | 0.21584 |
Rwork | 0.214 |
R-free | 0.25817 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | SEMET MODEL |
RMSD bond length | 0.019 |
RMSD bond angle | 1.566 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.700 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.039 | 0.240 |
Number of reflections | 16690 | |
<I/σ(I)> | 27.1 | 3.9 |
Completeness [%] | 98.9 | 92.3 |
Redundancy | 7.22 | 2.88 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 293 | MPD, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.50 |