1XVI

Crystal Structure of YedP, phosphatase-like domain protein from Escherichia coli K12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-30
Detector	SBC-3
Wavelength(s)	0.98016
Spacegroup name	P 1 21 1
Unit cell lengths	54.783, 79.975, 75.471
Unit cell angles	90.00, 99.56, 90.00

Refinement procedure

Resolution	74.330 - 2.260
R-factor	0.19
Rwork	0.184
R-free	0.24334
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.408
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.2.0000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.330	2.350
High resolution limit [Å]	2.260	2.260
Rmerge	0.060	0.219
Number of reflections	30085
<I/σ(I)>	7	3.9
Completeness [%]	99.4	95.4
Redundancy	4.1	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	PEG 8000, ammonium sulfate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	PEG 8000, ammonium sulfate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K