1XVI
Crystal Structure of YedP, phosphatase-like domain protein from Escherichia coli K12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-30 |
| Detector | SBC-3 |
| Wavelength(s) | 0.98016 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.783, 79.975, 75.471 |
| Unit cell angles | 90.00, 99.56, 90.00 |
Refinement procedure
| Resolution | 74.330 - 2.260 |
| R-factor | 0.19 |
| Rwork | 0.184 |
| R-free | 0.24334 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.408 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.330 | 2.350 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.060 | 0.219 |
| Number of reflections | 30085 | |
| <I/σ(I)> | 7 | 3.9 |
| Completeness [%] | 99.4 | 95.4 |
| Redundancy | 4.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | PEG 8000, ammonium sulfate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | PEG 8000, ammonium sulfate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
