1XV2
Crystal Structure of a Protein of Unknown Function Similar to Alpha-acetolactate Decarboxylase from Staphylococcus aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-15 |
Detector | SBC-2 |
Wavelength(s) | 0.979562 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.434, 160.005, 68.056 |
Unit cell angles | 90.00, 107.55, 90.00 |
Refinement procedure
Resolution | 30.240 - 2.000 |
R-factor | 0.191 |
Rwork | 0.191 |
R-free | 0.23600 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Phasing software | HKL-3000 |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.067 | |
Number of reflections | 126804 | |
Completeness [%] | 93.7 | |
Redundancy | 2 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.20M lithium sulphate, 0.10M tris pH 8.5, 25% polyethylene glycol 3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K |