1XU8
The 2.8 A structure of a tumour suppressing serpin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.540, 100.480, 136.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.22409 |
| Rwork | 0.221 |
| R-free | 0.28568 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1OVA CHAIN A |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.280 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.088 | 0.322 |
| Number of reflections | 18174 | |
| <I/σ(I)> | 11.5 | 3.2 |
| Completeness [%] | 96.9 | 93.7 |
| Redundancy | 2.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 295 | Ammonium sulfate, Bis-Tris, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
