1XTZ
Crystal structure of the S. cerevisiae D-ribose-5-phosphate isomerase: comparison with the archeal and bacterial enzymes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 209.240, 209.240, 209.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.100 |
| R-factor | 0.19529 |
| Rwork | 0.193 |
| R-free | 0.24733 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lk5 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.590 |
| Data scaling software | XDS |
| Phasing software | BEAST |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 23476 | |
| <I/σ(I)> | 19 | 6.4 |
| Completeness [%] | 100 | |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2M Magnesium Chloride, 30% Polyethylene Glycol 4000, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
