1XTE
crystal structure of CISK-PX domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2002-08-19 |
Detector | ANL-ECD |
Wavelength(s) | 0.97934 |
Spacegroup name | I 4 |
Unit cell lengths | 74.954, 74.954, 51.697 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.560 - 1.600 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.22000 |
Structure solution method | MAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.900 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.199 | |
Number of reflections | 18760 | |
<I/σ(I)> | 34 | 6.5 |
Completeness [%] | 98.6 | 97.7 |
Redundancy | 5.4 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | sodium citrate, sodium malonate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | sodium citrate, sodium malonate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |