1XTE
crystal structure of CISK-PX domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2002-08-19 |
| Detector | ANL-ECD |
| Wavelength(s) | 0.97934 |
| Spacegroup name | I 4 |
| Unit cell lengths | 74.954, 74.954, 51.697 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.560 - 1.600 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.22000 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.199 | |
| Number of reflections | 18760 | |
| <I/σ(I)> | 34 | 6.5 |
| Completeness [%] | 98.6 | 97.7 |
| Redundancy | 5.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | sodium citrate, sodium malonate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | sodium citrate, sodium malonate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
