1XTB
Crystal Structure of Rabbit Phosphoglucose Isomerase Complexed with Sorbitol-6-Phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2003-12-10 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9794 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 82.770, 115.190, 271.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 2.000 |
R-factor | 0.201 |
Rwork | 0.199 |
R-free | 0.21900 |
Structure solution method | isomorphous replacement |
Data reduction software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.073 | |
Number of reflections | 84679 | |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 298 | 16% PEG 8000, 250mM magnesium acetate, 100mM sodium citrate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |