1XT4
Urate Oxidase From Aspergillus Flavus Complexed With Guanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-11-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 79.984, 96.445, 105.543 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.010 |
| Rwork | 0.169 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r51 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.210 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.150 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 26240 | |
| <I/σ(I)> | 13.5 | 9.4 |
| Completeness [%] | 94.1 | 90.6 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 8.5MG/ML PROTEIN, 0.2MG/ML Guanine, 5-7%(W/V) PEG 8000, 100MM TRIS/HCL, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
