1XT3
Structure Basis of Venom Citrate-Dependent Heparin Sulfate-Mediated Cell Surface Retention of Cobra Cardiotoxin A3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL17B2 |
| Synchrotron site | NSRRC |
| Beamline | BL17B2 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.1274 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 43.903, 59.447, 98.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.400 |
| R-factor | 0.231 |
| Rwork | 0.227 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 4889 | |
| Completeness [%] | 96.8 | 83.6 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | PEG4000, isopropanol, trisodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
