1XSS
Semi-rational engineering of a green-emitting coral fluorescent protein into an efficient highlighter.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2003-06-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.7 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.740, 119.080, 49.290 |
| Unit cell angles | 90.00, 120.72, 90.00 |
Refinement procedure
| Resolution | 14.500 - 1.600 |
| R-factor | 0.197 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| <I/σ(I)> | 4.3 | |
| Completeness [%] | 95.0 | 94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | NaCl, HEPES , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
