1XSO

THREE-DIMENSIONAL STRUCTURE OF XENOPUS LAEVIS CU,ZN SUPEROXIDE DISMUTASE B DETERMINED BY X-RAY CRYSTALLOGRAPHY AT 1.5 ANGSTROMS RESOLUTION

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Detector technology	IMAGE PLATE
Collection date	1993-08-18
Detector	CUSTOM-MADE
Spacegroup name	P 21 21 21
Unit cell lengths	73.450, 68.940, 58.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.490
R-factor	0.104
Rwork	0.104
R-free	0.16900
RMSD bond length	0.022
RMSD bond angle	0.030 *
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	SHELXL-93

Data quality characteristics

	Overall
High resolution limit [Å]	1.490 *
Rmerge	0.078
Total number of observations	245181 *
Number of reflections	49209
Completeness [%]	98.8
Redundancy	4.98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6 *	301 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG4000	18 (%(w/v))
3	1	reservoir	sodium phosphate	0.05 (M)