1XSD
Crystal structure of the BlaI repressor in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-05 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.1000 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 72.141, 72.141, 243.472 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.237 |
| Rwork | 0.230 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1okr |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.700 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.065 | 0.546 |
| Number of reflections | 9285 | |
| <I/σ(I)> | 44 | 4.5 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 13.7 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 8000, ethylene glycol, magnesium chloride, sodium HEPES, potasssium phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
