1XS6
dCTP deaminase from Escherichia coli. E138A mutant enzyme in complex with dUTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-05-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.930, 63.073, 96.363 |
| Unit cell angles | 90.00, 102.18, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| R-factor | 0.26088 |
| Rwork | 0.258 |
| R-free | 0.31288 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xs1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.593 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 71843 | |
| <I/σ(I)> | 8 | 2.6 |
| Completeness [%] | 97.4 | 95.5 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 400, magnesium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
