1XRV
Crystal Structure of the novel secretory signalling protein from Porcine (SPP-40) at 2.1A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-09-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5412 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.588, 66.551, 107.466 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.800 - 2.100 |
| R-factor | 0.18984 |
| Rwork | 0.188 |
| R-free | 0.22753 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xhg |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.877 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.800 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 24978 | |
| <I/σ(I)> | 11 | 2.7 |
| Completeness [%] | 97.1 | 95.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 25mM tris-HCl, 50mM NaCl, 20% ethanol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
