1XRS
Crystal structure of Lysine 5,6-Aminomutase in complex with PLP, cobalamin, and 5'-deoxyadenosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-30 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 99.700, 99.700, 168.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.850 - 2.800 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.26200 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.850 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 24342 | |
| <I/σ(I)> | 25 | 9.1 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 12.8 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 298 | 24% (Wt/Vol) PEG 2000 MONOMETHYL ETHER, 0.1 M TRIS HYDROCHLORIDE, 0.02 M SODIUM ACETATE, PH 8.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |
